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non small cell lung cancer cell lines nci h460  (ATCC)


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    ATCC non small cell lung cancer cell lines nci h460
    Non Small Cell Lung Cancer Cell Lines Nci H460, supplied by ATCC, used in various techniques. Bioz Stars score: 98/100, based on 2637 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/non+small+cell+lung+cancer+cell+lines+nci+h460/NCI-H460/pmc13012240-33-16-57
    Average 98 stars, based on 2637 article reviews
    non small cell lung cancer cell lines nci h460 - by Bioz Stars, 2026-10
    98/100 stars

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    Cell Culture:

    Article Title: DAL-1/4.1B contributes to epithelial-mesenchymal transition via regulation of transforming growth factor-β in lung cancer cell lines.
    Article Snippet: .. The human non-small cell lung cancer cell lines NCI H460 and A549 cells were obtained from the American Type Culture Collection (Manassas, VA, USA) were cultured in RPMI-1640 medium (Gibco-BRL, Invitrogen Life Technologies, Carlsbad, CA, USA) supplemented with 10% fetal bovine serum (FBS; Hyclone, Logan, UT, USA) in the absence of antibiotics at 37 ̊C in a humidified atmosphere containing 5% CO2. .. The medium of actively growing cells was replenished with medium containing 10 ng/ml TGF-β (R&D Systems, Minneapolis, MN, USA).

    Article Title: KDM1A, a potent and selective target, for the treatment of DNMT3A-deficient non-small cell lung cancer.
    Article Snippet: BACKGROUND: DNMT3A is a crucial epigenetic regulation enzyme.. However, due to its heterogeneous nature and frequent mutation in various cancers, the role of DNMT3A remains controversial.. Here, we determine the role of DNMT3A in non-small cell lung cancer (NSCLC) to identify potential treatment strategies.



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    Image Search Results


    Illustration of the cytotoxicity activity of Ethyl 2-[5-(4-chlorophenyl)-2-methyl-1-H-Imidazole-4-yl] acetate in (A) A549 and (B) NCI-H460 cell lines.

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: Illustration of the cytotoxicity activity of Ethyl 2-[5-(4-chlorophenyl)-2-methyl-1-H-Imidazole-4-yl] acetate in (A) A549 and (B) NCI-H460 cell lines.

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Activity Assay

    SIRT6 expression in A549 and NCI-H460 NSCLC cell lines: (A) Represents the qRT-PCR analysis and (B) Represents the Western blot analysis. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. ** p < 0.01, *** p < 0.001 and **** p < 0.0001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: SIRT6 expression in A549 and NCI-H460 NSCLC cell lines: (A) Represents the qRT-PCR analysis and (B) Represents the Western blot analysis. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. ** p < 0.01, *** p < 0.001 and **** p < 0.0001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Expressing, Quantitative RT-PCR, Western Blot, Gene Expression, Control

    The effect of imidazole derivative Ethyl 2-[5-(4-chlorophenyl)-2-methyl-1-H-Imidazole-4-yl] acetate on antioxidant regulators (A) GSH Activity, (B) GPx Activity, (C) Catalase Activity, and (D) % Radical Scavenging Activity in A549 and NCI-H460 cell lines. (Data represent mean values ±SD. * p < 0.05, ** p < 0.01, and *** p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: The effect of imidazole derivative Ethyl 2-[5-(4-chlorophenyl)-2-methyl-1-H-Imidazole-4-yl] acetate on antioxidant regulators (A) GSH Activity, (B) GPx Activity, (C) Catalase Activity, and (D) % Radical Scavenging Activity in A549 and NCI-H460 cell lines. (Data represent mean values ±SD. * p < 0.05, ** p < 0.01, and *** p < 0.001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Activity Assay

    The protein expression of cytochrome-c (cyt-c) in NSCLC cell lines A549 and NCI-H460. β-actin is used as an internal control for normalization. (Data represent mean values ±SD. ** p < 0.01, and *** p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: The protein expression of cytochrome-c (cyt-c) in NSCLC cell lines A549 and NCI-H460. β-actin is used as an internal control for normalization. (Data represent mean values ±SD. ** p < 0.01, and *** p < 0.001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Expressing, Control

    Gene and protein expression of caspase 9: (A) Caspase 9 gene expression, (B) pro-caspase 9, and (C) active-caspase 9 protein expression in A549 and NCI-H460 cell lines. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization. (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: Gene and protein expression of caspase 9: (A) Caspase 9 gene expression, (B) pro-caspase 9, and (C) active-caspase 9 protein expression in A549 and NCI-H460 cell lines. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization. (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Expressing, Gene Expression, Control

    The expression of Caspase 3: (A) Caspase 3 gene expression, (B) protein expression of pro-caspase 3, and (C) active-caspase 3 in A549 and NCI-H460 cell lines. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD.** p < 0.01, *** p < 0.001 and **** p < 0.0001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: The expression of Caspase 3: (A) Caspase 3 gene expression, (B) protein expression of pro-caspase 3, and (C) active-caspase 3 in A549 and NCI-H460 cell lines. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD.** p < 0.01, *** p < 0.001 and **** p < 0.0001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Expressing, Gene Expression, Control

    Morphological assessment of NSCLC cell lines A549 and NCI-H460 cells stained by (A) AO/EtBr, (B) Propidium iodide, (C) DCFH-DA, (D) Rhodamine-123, and (E) Hoechst 33258 were captured using a fluorescent microscope with ×20 magnification (Scale Bar - 125 μm). (F) AO/EtBr, (G) Propidium iodide, (H) DCFH-DA, (I) Rhodamine-123, and (J) Hoechst 33258 represent the analysis of morphological staining by image (J) (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: Morphological assessment of NSCLC cell lines A549 and NCI-H460 cells stained by (A) AO/EtBr, (B) Propidium iodide, (C) DCFH-DA, (D) Rhodamine-123, and (E) Hoechst 33258 were captured using a fluorescent microscope with ×20 magnification (Scale Bar - 125 μm). (F) AO/EtBr, (G) Propidium iodide, (H) DCFH-DA, (I) Rhodamine-123, and (J) Hoechst 33258 represent the analysis of morphological staining by image (J) (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Staining, Microscopy

    Gene and protein expression of Keap1 in A549 and NCI-H460 NSCLC cell lines: (A) Keap1 gene expression and (B) Western blot analysis of Keap1. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. ** p < 0.01 and *** p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: Gene and protein expression of Keap1 in A549 and NCI-H460 NSCLC cell lines: (A) Keap1 gene expression and (B) Western blot analysis of Keap1. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. ** p < 0.01 and *** p < 0.001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Expressing, Gene Expression, Western Blot, Control

    Gene and protein expression of Nrf2 in A549 and NCI-H460 NSCLC cell lines: (A) Nrf2 gene expression and (B) Western blot analysis of Nrf2. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

    Journal: Frontiers in Pharmacology

    Article Title: Ameliorative inhibition of sirtuin 6 by imidazole derivative triggers oxidative stress-mediated apoptosis associated with Nrf2/Keap1 signaling in non-small cell lung cancer cell lines

    doi: 10.3389/fphar.2023.1335305

    Figure Lengend Snippet: Gene and protein expression of Nrf2 in A549 and NCI-H460 NSCLC cell lines: (A) Nrf2 gene expression and (B) Western blot analysis of Nrf2. GAPDH for gene expression and β-actin for protein expression were used as an internal control for normalization (Data represent mean values ±SD. * p < 0.05, ** p < 0.01 and *** p < 0.001).

    Article Snippet: The human non-small cell lung cancer cell lines A549 (Adenocarcinoma) and NCI-H460 (large cell carcinoma) were purchased from the National Centre for Cell Science (NCCS), Pune, India.

    Techniques: Expressing, Gene Expression, Western Blot, Control